Difference between revisions of "Proteomics"
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(Created page with "== Methods for Proteomics Research == Sample preparation * Isolation of muscle protein & depletion of acto-myosin components (S. Lange)") |
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== Methods for Proteomics Research == | == Methods for Proteomics Research == | ||
+ | === Protein Analysis === | ||
+ | * [[SDS-page gels|Preparing SDS-page gels]] | ||
+ | * [[Ponceau Staining of Protein Blots]] | ||
+ | *[[Preparing Primary Antibodies for Immunoblots]] | ||
+ | *[[Preparing Secondary Antibodies for Immunoblots]] | ||
+ | *[[Developing Your Immunoblot Membrane]] | ||
+ | *[[Pierce BCA Protein Concentration]] | ||
− | Sample preparation | + | ===Creating Western Blot Solutions=== |
+ | *[[Creating a Lower Buffer Solution]] | ||
+ | |||
+ | === Sample preparation === | ||
* [[Isolation of muscle protein & depletion of acto-myosin components]] (S. Lange) | * [[Isolation of muscle protein & depletion of acto-myosin components]] (S. Lange) |
Latest revision as of 01:00, 31 July 2018
Contents
Methods for Proteomics Research
Protein Analysis
- Preparing SDS-page gels
- Ponceau Staining of Protein Blots
- Preparing Primary Antibodies for Immunoblots
- Preparing Secondary Antibodies for Immunoblots
- Developing Your Immunoblot Membrane
- Pierce BCA Protein Concentration