Proteomics

Proteomics

Methods for Proteomics Research

Protein Analysis

• Preparing SDS-page gels
• Ponceau Staining of Protein Blots
• Preparing Primary Antibodies for Immunoblots
• Preparing Secondary Antibodies for Immunoblots
• Developing Your Immunoblot Membrane
• Pierce BCA Protein Concentration

Creating Western Blot Solutions

• Creating a Lower Buffer Solution

Sample preparation

• Isolation of muscle protein & depletion of acto-myosin components (S. Lange)