User contributions
(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 16:10, 31 July 2018 (diff | hist) . . (+1,865) . . N Pierce BCA Protein Concentration (Created page with "==Summary== We use this method measure the amount of protein we have in our unknown samples ===Method=== # *Place samples in order on ice* # Use the sonicator to break up all...") (current)
- 01:00, 31 July 2018 (diff | hist) . . (+38) . . Proteomics (current)
- 00:59, 31 July 2018 (diff | hist) . . (+1,714) . . N Transfecting C2C12 Cells (Created page with "=== Transfecting C2C12 Cells=== * siRNA against Cullin 3 * siRNA with other sequence ===Materials=== * Two tubes (1.7ml) per well (12 tubes total) * 6 for control * 6 for tr...") (current)
- 00:44, 31 July 2018 (diff | hist) . . (+30) . . Cell Biology
- 20:29, 23 July 2018 (diff | hist) . . (-12) . . Seeding C2C12 Cells (current)
- 20:27, 23 July 2018 (diff | hist) . . (+22) . . Freezing C2C12 and Satellite Cells (current)
- 20:25, 23 July 2018 (diff | hist) . . (+13) . . Designing Oligonucleotides for qPCR (current)
- 20:24, 23 July 2018 (diff | hist) . . (+13) . . Designing Oligonucleotides for qPCR
- 20:24, 23 July 2018 (diff | hist) . . (+53) . . Scanning PCR Gel (current)
- 20:20, 23 July 2018 (diff | hist) . . (+33) . . Creating a Lower Buffer Solution (current)
- 20:18, 23 July 2018 (diff | hist) . . (-26) . . Developing Your Immunoblot Membrane (current)
- 20:18, 23 July 2018 (diff | hist) . . (+66) . . Preparing Primary Antibodies for Immunoblots (current)
- 20:18, 23 July 2018 (diff | hist) . . (+1) . . Preparing Secondary Antibodies for Immunoblots (current)
- 20:17, 23 July 2018 (diff | hist) . . (-49) . . Preparing Primary Antibodies for Immunoblots
- 20:16, 23 July 2018 (diff | hist) . . (-38) . . Ponceau Staining of Protein Blots (current)
- 20:15, 23 July 2018 (diff | hist) . . (+12) . . SDS-page gels (current)
- 20:12, 23 July 2018 (diff | hist) . . (+1,153) . . N Measuring RNA Concentration (Created page with "===Summary=== In order to quantify the concentration of RNA we have in our sample we use a Nanodrop * Nanodrop is located in the hallway * Need kimwipes and milli Q water (M...") (current)
- 20:07, 23 July 2018 (diff | hist) . . (+34) . . Cell Biology
- 20:06, 23 July 2018 (diff | hist) . . (-25) . . Extraction of RNA (current)
- 15:43, 23 July 2018 (diff | hist) . . (+3,260) . . N Extraction of RNA (Created page with "===Extracting RNA post stimulation and collection=== '''EXAMPLE:''' Starting w/ four samples (before, 6hr, 12hrs, 24hrs) # Use the hood in the hallway, remove any stuff that...")
- 15:42, 23 July 2018 (diff | hist) . . (+23) . . Cell Biology
- 15:41, 23 July 2018 (diff | hist) . . (+830) . . N Creating a Lower Buffer Solution (Created page with "===Making 1.5M Lower Buffer Solution=== *500 total to make # 91g of tris base (into a beaker with a stir bar) # 350 ml of milli Q (from the tap not reservoir) measure in a gr...")
- 15:40, 23 July 2018 (diff | hist) . . (+77) . . Proteomics
- 15:37, 23 July 2018 (diff | hist) . . (+18) . . Molecular Biology (current)
- 15:33, 23 July 2018 (diff | hist) . . (+1,099) . . Designing Oligonucleotides for qPCR
- 15:29, 23 July 2018 (diff | hist) . . (+376) . . SDS-page gels
- 21:30, 20 July 2018 (diff | hist) . . (+20) . . Transfecting Cells (current)
- 21:27, 20 July 2018 (diff | hist) . . (+12) . . Transfecting Cells
- 21:27, 20 July 2018 (diff | hist) . . (-416) . . Transfecting Cells
- 21:27, 20 July 2018 (diff | hist) . . (-1) . . Transfecting Cells
- 21:27, 20 July 2018 (diff | hist) . . (+385) . . Transfecting Cells
- 21:23, 20 July 2018 (diff | hist) . . (+1,523) . . N Transfecting Cells (Created page with "==Summary== Cos 1 (African green monkey, from kidney cells). Cells are heterozygous, they all look different. We are transfecting Cos 1, 6 cm plates * Make sure everything is...")
- 21:21, 20 July 2018 (diff | hist) . . (+24) . . Cell Biology
- 21:16, 20 July 2018 (diff | hist) . . (+1,335) . . N Designing Oligonucleotides for qPCR (Created page with "==Summary== We design primers get an optimal PCR. It is an important step. If you use any primers without design, the amplification may not work or give you skewed results =...")
- 21:06, 20 July 2018 (diff | hist) . . (+41) . . Molecular Biology
- 21:04, 20 July 2018 (diff | hist) . . (+2,305) . . N Seeding C2C12 Cells (Created page with "==Seeding C2C12 cells== Check how confluent cell dishes are. Mark the one that will be used for seeding (if there is more than one dish) choose the medium confluent dish and t...")
- 20:20, 19 July 2018 (diff | hist) . . (+25) . . Cell Biology
- 20:18, 19 July 2018 (diff | hist) . . (+1,451) . . N Collection of Proteins and RNA Post- Stimulation (Created page with "===Collecting proteins at each stimulation time point=== # Warm solutions needed (PBS, lysis buffer) # Clean cell scrapers in DI water, place in hood # Collect ice for the sam...") (current)
- 20:17, 19 July 2018 (diff | hist) . . (+55) . . Cell Biology
- 20:13, 19 July 2018 (diff | hist) . . (+250) . . Stimulation of C2C12 Cells with Neural Agrin (current)
- 20:04, 19 July 2018 (diff | hist) . . (+1,065) . . N Stimulation of C2C12 Cells with Neural Agrin (Created page with "==Stimulation of C2C12 cells with Agrin== As mentioned before: -PBS is the control (found in the freezer, bottom shelf, agrin box, PBS 0.1% BSA 1000x) -Neural agrin (x1000) is...")
- 20:02, 19 July 2018 (diff | hist) . . (+51) . . Cell Biology
- 20:02, 19 July 2018 (diff | hist) . . (+2) . . Scanning PCR Gel
- 20:01, 19 July 2018 (diff | hist) . . (-9) . . Scanning PCR Gel
- 20:01, 19 July 2018 (diff | hist) . . (+589) . . N Scanning PCR Gel (Created page with "==Scanning PCR gel== # After the gel is done running, disconnect the power source and remove gel onto a piece of paper or a paper towel # Dry the gel a little and place into t...")
- 20:00, 19 July 2018 (diff | hist) . . (+22) . . Molecular Biology
- 19:59, 19 July 2018 (diff | hist) . . (+2) . . Genotyping PCR (current)
- 19:58, 19 July 2018 (diff | hist) . . (+77) . . Genotyping PCR
- 18:00, 19 July 2018 (diff | hist) . . (+30) . . Genotyping PCR
- 22:57, 18 July 2018 (diff | hist) . . (+2,344) . . N Genotyping PCR (Created page with "==Summary== DNA is amplified by three steps: denaturation, annealing, and elongation. The wild type control (WT) is negative for both CRE and Cullin 3 CRE positive and cullin...")
(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
