Center Cores & Technologies - Imaging Core

The Imaging Cores in San Diego provide both fluorescence light microscopy and electron microscopy resources to enable evaluation of muscle morphology and structural organization, and to localize specific molecular components in the sarcomeres, cytoskeleton, subcellular organelles and membranes. The two imaging cores can assist investigators in fluorescence light microscopy and transmission electron microscopy analyses of muscle tissues and cells isolated from wild-type and transgenic mice, or from healthy and diseased human muscles. The Cores support investigators with training and assistance in the complex imaging technologies of Transmission Electron Microscopy and Confocal Laser Scanning Fluorescence Microscopy on fixed specimens, Confocal Spinning Disc and Wide-Field Fluorescence Microscopy of molecular dynamics in living cells, and Single-Molecule Fluorescence imaging using Total Internal Reflection Fluorescence (TIRF) Microscopy. The Cores will provide training and assistance with routine aspects of sample preparation for microscopy, and training and access to microscopes in The Scripps Research Institute (TSRI) Microscopy Facility and the UC San Diego Microscopy Core facility. The Cores will also assist investigators in application of image analysis software to their experimental problems and in quantitative interpretation of fluorescent image data.

Imaging Cores in San Diego

The services of the imaging core have been merged into the Core Microscopy Facility at Scripps and the UC San Diego Microscopy Core facility.