User contributions
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- 22:54, 18 July 2018 (diff | hist) . . (+20) . . Molecular Biology
- 22:52, 18 July 2018 (diff | hist) . . (-43) . . Making 2% Agarose Gel for PCR Analysis (current)
- 22:52, 18 July 2018 (diff | hist) . . (+906) . . N Making 2% Agarose Gel for PCR Analysis (Created page with "==Making 2% Agarose Gel for PCR Analysis== ===Materials=== * Agarose powder (kept in cabinet) * 1x TAE liquid (by sink) ===Methods=== # Start by taping the gel mold (found b...")
- 22:51, 18 July 2018 (diff | hist) . . (-43) . . Making 4% Agarose Gel for PCR Analysis (current)
- 22:50, 18 July 2018 (diff | hist) . . (+907) . . N Making 4% Agarose Gel for PCR Analysis (Created page with "==Making 4% Agarose Gel for PCR Analysis== ===Materials=== * Agarose powder (kept in cabinet) * 1x TAE liquid (by sink) ===Methods=== # Start by taping the gel mold (found b...")
- 22:50, 18 July 2018 (diff | hist) . . (+44) . . Molecular Biology
- 22:49, 18 July 2018 (diff | hist) . . (+906) . . N Making 2% Agarose Gel for PCR analysis (Created page with "==Making 2% Agarose Gel for PCR Analysis== ===Materials=== * Agarose powder (kept in cabinet) * 1x TAE liquid (by sink) ===Methods=== # Start by taping the gel mold (found b...") (current)
- 22:47, 18 July 2018 (diff | hist) . . (0) . . Freezing C2C12 and Satellite Cells
- 22:46, 18 July 2018 (diff | hist) . . (+1,811) . . N Freezing C2C12 and Satellite Cells (Created page with "==Freezing C2C12 and Satellite cells== ===Methods=== * Mr. Freeze box with isopentane * Cryotube (in tissue culture box) * Warm PBS, basic FGF *Trypsin * Gelatin coated dish...")
- 22:42, 18 July 2018 (diff | hist) . . (+41) . . Cell Biology
- 22:39, 18 July 2018 (diff | hist) . . (-1) . . Creating Cell Culture Media (current)
- 22:39, 18 July 2018 (diff | hist) . . (+1,261) . . N Creating Cell Culture Media (Created page with "==How to Make Cell Culture Media for C2C12 and Satellite Cells== ===Differentiation Media for C2C12 cells=== * 200 ml DMEM * 4 ml horse serum (2%) * 3 ml penicillin/ streptomy...")
- 22:32, 18 July 2018 (diff | hist) . . (+34) . . Cell Biology
- 22:32, 18 July 2018 (diff | hist) . . (-2) . . Passaging of C2C12 cells (current)
- 22:30, 18 July 2018 (diff | hist) . . (+902) . . N Passaging of C2C12 cells (Created page with "==Making a New Passage C2C12 cells== ===Summary=== We passage cells to keep them alive and growing in the incubator in the cell culture room. Generally cells should be passage...")
- 22:17, 18 July 2018 (diff | hist) . . (+339) . . Changing Proliferation Media to Differentiation Media (current)
- 22:17, 18 July 2018 (diff | hist) . . (-337) . . Changing Proliferation Media to Differentiation Media
- 22:17, 18 July 2018 (diff | hist) . . (-92) . . Changing Proliferation Media to Differentiation Media
- 22:17, 18 July 2018 (diff | hist) . . (+93) . . Changing Proliferation Media to Differentiation Media
- 22:16, 18 July 2018 (diff | hist) . . (+638) . . N Changing Proliferation Media to Differentiation Media (Created page with "==C2C12 - Changing Proliferation Media to Differentiation Media== ===Summary=== In order to continue myotube formation 'in vitro' we must change the proliferation medium (afte...")
- 22:12, 18 July 2018 (diff | hist) . . (0) . . Cell Biology
- 22:11, 18 July 2018 (diff | hist) . . (+1,985) . . N Developing Your Immunoblot Membrane (Created page with "==Developing your Immunoblot Membrane== ===Materials=== *A flash drive *A tube rack *Sheet protectors *PICO developing solution *15 ml tube *Ethanol + KimWipes towels # Afte...")
- 21:59, 18 July 2018 (diff | hist) . . (+41) . . Proteomics
- 21:55, 18 July 2018 (diff | hist) . . (+36) . . Preparing Secondary Antibodies for Immunoblots
- 21:54, 18 July 2018 (diff | hist) . . (+1,970) . . N Preparing Secondary Antibodies for Immunoblots (Created page with "==Preparing Secondary Antibodies for Immunoblots== ===Summary=== As mentioned the secondary antibody is used to visualize what you are looking for, it is an amplificator. We a...")
- 21:52, 18 July 2018 (diff | hist) . . (+52) . . Proteomics
- 21:51, 18 July 2018 (diff | hist) . . (+14) . . Ponceau Staining of Protein Blots
- 21:49, 18 July 2018 (diff | hist) . . (+13) . . Preparing Primary Antibodies for Immunoblots
- 21:49, 18 July 2018 (diff | hist) . . (-1) . . Proteomics
- 21:48, 18 July 2018 (diff | hist) . . (+18) . . Preparing Primary Antibodies for Immunoblots
- 21:20, 18 July 2018 (diff | hist) . . (-1) . . Preparing Primary Antibodies for Immunoblots
- 21:20, 18 July 2018 (diff | hist) . . (+2,140) . . N Preparing Primary Antibodies for Immunoblots (Created page with "==Preparing Primary Antibodies for Immunoblot== ===Summary=== Make sure the proteins you are identifying are not the same molecular weight. Antibodies are important because...")
- 21:19, 18 July 2018 (diff | hist) . . (+51) . . Proteomics
- 21:16, 18 July 2018 (diff | hist) . . (+2,388) . . N Ponceau Staining of Protein Blots (Created page with "==Ponceau Staining of Protein Blots== ===Summary=== In order to ensure the transfer went through you will stain your membrane in ponceau solution. ===Materials=== * Pre-made...")
- 21:13, 18 July 2018 (diff | hist) . . (0) . . Proteomics
- 21:12, 18 July 2018 (diff | hist) . . (+2,388) . . N Ponceau staining of protein blots (Created page with "==Ponceau Staining of Protein Blots== ===Summary=== In order to ensure the transfer went through you will stain your membrane in ponceau solution. ===Materials=== * Pre-made...") (current)
- 21:04, 18 July 2018 (diff | hist) . . (+40) . . Proteomics
- 21:01, 18 July 2018 (diff | hist) . . (+1,423) . . N Loading a Protein Gel (Created page with "(Identify how much protein you will have to load in the wells (excel sheet)) # Take the gel apparatus and place it onto the running adapter (positive and negative screws) make...") (current)
- 21:00, 18 July 2018 (diff | hist) . . (+324) . . N Running a Protein Gel (Created page with "# Connect (green) power top onto the running adapter. Ensure the screws are on tight and top matches with screws: black to black, red to red. # Set power supply to 100 volts...") (current)
- 20:58, 18 July 2018 (diff | hist) . . (-1,373) . . Transfering a Protein Gel (Blanked the page) (current)
- 20:57, 18 July 2018 (diff | hist) . . (+1,373) . . N Transferring a Protein Gel (Created page with "# Once run is complete (blue is running out of gel) switch off power supply # Place transfer buffer into a container (found on drying rack) '' Transfer buffer: 28.8g glycine,...") (current)
- 20:57, 18 July 2018 (diff | hist) . . (-1,410) . . SDS-page gels
- 20:56, 18 July 2018 (diff | hist) . . (+1,412) . . SDS-page gels
- 20:56, 18 July 2018 (diff | hist) . . (+1,410) . . SDS-page gels
- 20:56, 18 July 2018 (diff | hist) . . (-1,410) . . SDS-page gels
- 20:55, 18 July 2018 (diff | hist) . . (-1,410) . . SDS-page gels
- 20:55, 18 July 2018 (diff | hist) . . (+1,410) . . SDS-page gels
- 20:53, 18 July 2018 (diff | hist) . . (-31) . . Transfering a Protein Gel
- 20:53, 18 July 2018 (diff | hist) . . (+1,404) . . N Transfering a Protein Gel (Created page with "==Transferring a Protein Gel== # Once run is complete (blue is running out of gel) switch off power supply # Place transfer buffer into a container (found on drying rack) ''...")
- 20:50, 18 July 2018 (diff | hist) . . (+47) . . SDS-page gels
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