Difference between revisions of "Creating a Lower Buffer Solution"
From SDMRC
(Created page with "===Making 1.5M Lower Buffer Solution=== *500 total to make # 91g of tris base (into a beaker with a stir bar) # 350 ml of milli Q (from the tap not reservoir) measure in a gr...") |
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===Making 1.5M Lower Buffer Solution=== | ===Making 1.5M Lower Buffer Solution=== | ||
| − | * | + | *500ml total to make |
| − | + | ===Materials=== | |
| − | + | * 91g of tris base (into a beaker with a stir bar) | |
| + | * 350 ml of milli Q (from the tap not reservoir) measure in a graduated cylinder | ||
| + | |||
| + | ===Method=== | ||
# Use pH meter and temp sensor (clean with DI water) | # Use pH meter and temp sensor (clean with DI water) | ||
# Place pH electrode into beaker after calibrating | # Place pH electrode into beaker after calibrating | ||
Latest revision as of 20:20, 23 July 2018
Making 1.5M Lower Buffer Solution
- 500ml total to make
Materials
- 91g of tris base (into a beaker with a stir bar)
- 350 ml of milli Q (from the tap not reservoir) measure in a graduated cylinder
Method
- Use pH meter and temp sensor (clean with DI water)
- Place pH electrode into beaker after calibrating
- First pH will be around 11 (tris base is very basic)
- Add the temp sensor meter
- Hydrochloric acid (HCL) is used to set pH (be careful with the bottle it is coated, kept under the fume hood in the hallway)
- Use dropper to take up the HCL (tip bottle at 45 degrees) and check pH till 8.8, add HCL slowly as drops
- After pH is reached take on to the weighing bench (clean pH and temp sensor)
- Add 2g of 0.04% SDS (careful is powdery) dissolve on stir bar
- For the 1 M buffer: 700 ml of milli Q and 121g of tris base. pH is 7.4
