Changing Proliferation Media to Differentiation Media

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C2C12 - Changing Proliferation Media to Differentiation Media

Summary

In order to continue myotube formation 'in vitro' we must change the proliferation medium (after 24hrs) to differentiation media

Materials

  • Six well plates
  • Warmed differentiation media
  • 10ml serological pipettes

Method

  1. Warm solutions needed (diff. media)
  2. Remove all prolif. Media from old dish using two pipette tips and the vacuum
  3. Wash each cell well with 1 ml diff. Media and remove
  4. Add 2 ml of diff. Media to each cell well
  5. Place back into the incubator for five days. Make sure to label the plates with date/ cell type/ initials
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