SDS-page gels
From SDMRC
Contents
Creating a Protein Gel
Materials
- Clean casting stand w/ dual gel apparatus
- Glass plate holders (w/ white or black knobs)
- Additional spacers (1.5) with (1.5) size combs
- Different spacers can be used to create different gel widths (ie 0.75 spacers compared to 1.5 spacers)
- Two clean glass plates (one larger and one smaller, cleaned with ethanol + water)
- If a plate has a crack on it, crack can be sealed with clear tape
- Look out for cracks on the bottom of the plates, this will cause leaks
Steps for assembling gel apparatus
- Place the spacers onto the larger clean glass, by the edges, and place the smaller clean glass plate on top
- Place the two glass plates into the glass plate holder (knobs facing away from you) and adjust until bottom is flush and spacers are in the corners. Tighten the knobs
- Take the whole glass holder (w/ adjusted plates) onto the casting apparatus. Start by pushing the holder into the rubber gasket on the bottom of the casting apparatus and snap into the top portion
- To ensure the seal has been made, use a dropper to add a small portion of DI water to test. Test also the green combs to ensure the correct spacers are used
