Difference between revisions of "Measuring RNA Concentration"

Latest revision as of 20:12, 23 July 2018

Summary

In order to quantify the concentration of RNA we have in our sample we use a Nanodrop

• Nanodrop is located in the hallway
• Need kimwipes and milli Q water (MQ water is by the machine)
• The nanodrop program is called “nanodrop 2000” and its located on the desktop
• Use only 1 ul of solution on metal so use a small volume pipette

Using a nanodrop

1. Clean the nanodrop with MQ water, make sure to thoroughly dry
2. Click “nucleic acid”
3. Click “no”
4. Click “okay for verification”
5. Load blank solution (MQ water)
6. Set pipette to 1 ul and take up water. Place on sensor as a small drop in the middle of the sensor
7. Hit the “blank” button (only have to do blank once in the beginning
8. Clean with MQ water and dry
9. Measure the concentration of MQ water again to test (should give a value of zero)
10. Hit “measure”
11. “Save data in folder”
12. Repeat steps with each tube and save data. Change tips between each sample. Make sure program says “RNA”
13. Record concentrations in your lab book and on the tube
14. On the tube write “Ɣ (gamma) = ng/ul”
15. Also record the 260/280 and 260/230 counts