Difference between revisions of "Making 2% Agarose Gel for PCR Analysis"
From SDMRC
(Created page with "==Making 2% Agarose Gel for PCR Analysis== ===Materials=== * Agarose powder (kept in cabinet) * 1x TAE liquid (by sink) ===Methods=== # Start by taping the gel mold (found b...") |
(No difference)
|
Revision as of 22:52, 18 July 2018
Making 2% Agarose Gel for PCR Analysis
Materials
- Agarose powder (kept in cabinet)
- 1x TAE liquid (by sink)
Methods
- Start by taping the gel mold (found behind sink) , use three layers of tape each end
- Place in the gel mold combs, make sure they are all facing the same direction (knobs towards you)
- Create the gel solution in the agarose gel glass beaker
- 250 ml TAE
- 5g of agarose powder
- Microwave for two minutes, swirl carefully (make sure you have the rubber holder) if not dissolved microwave for another minute. Then let it cool
- Once dissolved and cooled add 25 ul of 10,000x DNA stain solution (kept in fridge door_brown tube) and swirl until orange color disappears
- Pour solution into agarose mold (in the hood) slowly from one side, and set to solidify (for a quick solid place in cold room)
- Once solid cut gels and store in black fridge in the gel container
