Difference between revisions of "Creating Cell Culture Media"
From SDMRC
(Created page with "==How to Make Cell Culture Media for C2C12 and Satellite Cells== ===Differentiation Media for C2C12 cells=== * 200 ml DMEM * 4 ml horse serum (2%) * 3 ml penicillin/ streptomy...") |
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# Attach the vacuum filter hose to increase filtration | # Attach the vacuum filter hose to increase filtration | ||
# Detach hose, label new bottle (name/date/ recipe) | # Detach hose, label new bottle (name/date/ recipe) | ||
| − | # Store in fridge | + | # Store in fridge |
===Proliferation Media for Satellite cells=== | ===Proliferation Media for Satellite cells=== | ||
Latest revision as of 22:39, 18 July 2018
Contents
How to Make Cell Culture Media for C2C12 and Satellite Cells
Differentiation Media for C2C12 cells
- 200 ml DMEM
- 4 ml horse serum (2%)
- 3 ml penicillin/ streptomycin
- Use a sterile vacuum filter (found on bench)
- In the hood, place all ingredients for the media in the top half of the filter
- Attach the vacuum filter hose to increase filtration
- Detach hose, label new bottle (name/date/ recipe)
- Store in fridge
Proliferation Media for C2C12 and Satellite cells
- 250 ml DMEM
- 50 ml FBS (fetal bovine serum- includes growth factors)
- 3 ml penicillin/streptomycin
- Use a sterile vacuum filter (found on bench)
- In the hood, place all ingredients for the media in the top half of the filter
- Attach the vacuum filter hose to increase filtration
- Detach hose, label new bottle (name/date/ recipe)
- Store in fridge
Proliferation Media for Satellite cells
- 250 ml Harms
- 50 ml FBS (fetal bovine serum- includes growth factors)
- 3 ml penicillin/ streptomycin
- Use a sterile vacuum filter (found on bench)
- In the hood, place all ingredients for the media in the top half of the filter
- Attach the vacuum filter hose to increase filtration
- Detach hose, label new bottle (name/date/ recipe)
- Store in fridge
