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| − | # Once run is complete (blue is running out of gel) switch off power supply
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| − | # Place transfer buffer into a container (found on drying rack)
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| − | '' Transfer buffer: 28.8g glycine, 6.07g tris base, 200 ml methanol''
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| − | # Build your sandwich in transfer buffer (two sandwiches)
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| − | * White side of sandwich holder down
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| − | * One black mesh pad
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| − | * 2 layers of filter paper
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| − | * One piece of nitrocellulose membrane (not PVDF) fitted to gel size
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| − | * Your gel
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| − | '''To remove gel:'''
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| − | take glass plates carefully out of the glass plate holder
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| − | Remove the smaller glass plate by slowly turning one spacer towards you, lifting the plate and making the gel stick to the larger plate
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| − | Take the larger plate into the solution with the sandwich and use a razor blade to carefully pry the gel from the larger glass plate
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| − | Once it is onto the membrane in solution, adjust so gel is centered on membrane
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| − | * 2 layers of filter paper
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| − | * One black mesh pad
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| − | # Black side of sandwich holder down, and close tight
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| − | # Place both sandwich holders into transfer apparatus with both black sides facing the black wall of the transfer apparatus. Put the entire apparatus into the transfer chamber
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| − | # Place an ice block (from the freezer) into the chamber and fill with transfer buffer to the top
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| − | # Place the chamber top on, and switch on the power supply
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| − | # Transfer the gel at 500 volts/ 200 milliamps on the bench, overnight
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