Difference between revisions of "Changing Proliferation Media to Differentiation Media"

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* Warmed differentiation media
 
* Warmed differentiation media
 
* 10ml serological pipettes
 
* 10ml serological pipettes
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==Method==
 
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# Warm solutions needed (diff. media)
 
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# Remove all prolif. Media from old dish using two pipette tips and the vacuum
 
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# Wash each cell well with 1 ml diff. Media and remove
 
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# Add 2 ml of diff. Media to each cell well
 
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# Place back into the incubator for five days. Make sure to label the plates with date/ cell type/ initials
 

Revision as of 22:17, 18 July 2018

C2C12 - Changing Proliferation Media to Differentiation Media

Summary

In order to continue myotube formation 'in vitro' we must change the proliferation medium (after 24hrs) to differentiation media

Materials

  • Six well plates
  • Warmed differentiation media
  • 10ml serological pipettes
Retrieved from "http://sdmrc.ucsd.edu/~sdmrcwiki/index.php?title=Changing_Proliferation_Media_to_Differentiation_Media&oldid=245"