Difference between revisions of "Changing Proliferation Media to Differentiation Media"
From SDMRC
| Line 7: | Line 7: | ||
* Warmed differentiation media | * Warmed differentiation media | ||
* 10ml serological pipettes | * 10ml serological pipettes | ||
| + | |||
| + | ===Method=== | ||
| + | # Warm solutions needed (diff. media) | ||
| + | # Remove all prolif. Media from old dish using two pipette tips and the vacuum | ||
| + | # Wash each cell well with 1 ml diff. Media and remove | ||
| + | # Add 2 ml of diff. Media to each cell well | ||
| + | # Place back into the incubator for five days. Make sure to label the plates with date/ cell type/ initials | ||
Latest revision as of 22:17, 18 July 2018
Contents
C2C12 - Changing Proliferation Media to Differentiation Media
Summary
In order to continue myotube formation 'in vitro' we must change the proliferation medium (after 24hrs) to differentiation media
Materials
- Six well plates
- Warmed differentiation media
- 10ml serological pipettes
Method
- Warm solutions needed (diff. media)
- Remove all prolif. Media from old dish using two pipette tips and the vacuum
- Wash each cell well with 1 ml diff. Media and remove
- Add 2 ml of diff. Media to each cell well
- Place back into the incubator for five days. Make sure to label the plates with date/ cell type/ initials
