Difference between revisions of "Changing Proliferation Media to Differentiation Media"
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(Created page with "==C2C12 - Changing Proliferation Media to Differentiation Media== ===Summary=== In order to continue myotube formation 'in vitro' we must change the proliferation medium (afte...") |
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==C2C12 - Changing Proliferation Media to Differentiation Media== | ==C2C12 - Changing Proliferation Media to Differentiation Media== | ||
===Summary=== | ===Summary=== | ||
| − | In order to continue myotube formation 'in vitro' we must change the proliferation medium (after 24hrs) to differentiation media | + | In order to continue myotube formation 'in vitro' we must change the proliferation medium (after 24hrs) to differentiation media |
| − | ==Materials== | + | ===Materials=== |
* Six well plates | * Six well plates | ||
* Warmed differentiation media | * Warmed differentiation media | ||
* 10ml serological pipettes | * 10ml serological pipettes | ||
| − | ==Method== | + | ===Method=== |
# Warm solutions needed (diff. media) | # Warm solutions needed (diff. media) | ||
# Remove all prolif. Media from old dish using two pipette tips and the vacuum | # Remove all prolif. Media from old dish using two pipette tips and the vacuum | ||
Latest revision as of 22:17, 18 July 2018
Contents
C2C12 - Changing Proliferation Media to Differentiation Media
Summary
In order to continue myotube formation 'in vitro' we must change the proliferation medium (after 24hrs) to differentiation media
Materials
- Six well plates
- Warmed differentiation media
- 10ml serological pipettes
Method
- Warm solutions needed (diff. media)
- Remove all prolif. Media from old dish using two pipette tips and the vacuum
- Wash each cell well with 1 ml diff. Media and remove
- Add 2 ml of diff. Media to each cell well
- Place back into the incubator for five days. Make sure to label the plates with date/ cell type/ initials
