View source for Creating a Lower Buffer Solution

===Making 1.5M  Lower Buffer Solution===
*500ml  total to make
===Materials===
* 91g of tris base (into a beaker with a stir bar)
* 350 ml of milli Q (from the tap not reservoir) measure in a graduated cylinder

===Method===
# Use pH meter and temp sensor (clean with DI water) 
# Place pH electrode into beaker after calibrating 
# First pH will be around 11 (tris base is very basic)
# Add the temp sensor meter
# Hydrochloric acid (HCL) is used to set pH (be careful with the bottle it is coated, kept under the fume hood in the hallway)
# Use dropper to take up the HCL (tip bottle at 45 degrees) and check pH till 8.8,  add HCL slowly as drops
# After pH is reached take on to the weighing bench (clean pH and temp sensor)
# Add 2g of 0.04% SDS (careful is powdery) dissolve on stir bar
*For the 1 M buffer: 700 ml of milli Q and 121g of tris base. pH is 7.4