Measuring RNA Concentration
From SDMRC
Summary
In order to quantify the concentration of RNA we have in our sample we use a Nanodrop
- Nanodrop is located in the hallway
- Need kimwipes and milli Q water (MQ water is by the machine)
- The nanodrop program is called “nanodrop 2000” and its located on the desktop
- Use only 1 ul of solution on metal so use a small volume pipette
Using a nanodrop
- Clean the nanodrop with MQ water, make sure to thoroughly dry
- Click “nucleic acid”
- Click “no”
- Click “okay for verification”
- Load blank solution (MQ water)
- Set pipette to 1 ul and take up water. Place on sensor as a small drop in the middle of the sensor
- Hit the “blank” button (only have to do blank once in the beginning
- Clean with MQ water and dry
- Measure the concentration of MQ water again to test (should give a value of zero)
- Hit “measure”
- “Save data in folder”
- Repeat steps with each tube and save data. Change tips between each sample. Make sure program says “RNA”
- Record concentrations in your lab book and on the tube
- On the tube write “Ɣ (gamma) = ng/ul”
- Also record the 260/280 and 260/230 counts
