Tail DNA extraction

Tail DNA extraction

Extraction of DNA from mouse tails

Method Summary

This method describes how to extract DNA from mouse tails. Please see also Alternative methods.

Materials

Lysis buffer

50mM NaOH

Neutralization buffer

1M Tris pH8

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Method

1. have each tail in a separate eppendorf tube
2. add 400μl of Lysis buffer to each tail and incubate for 20-30 mins. at approx. 95°C
3. (vortex 1-2 times in between to help dissolve tissue)
4. add 50µl of Neutralization buffer
5. vortex briefly
6. spin 1 minute max speed in tabletop centrifuge to collect bones and
7. remaining tissue (hairs) at bottom of the tube
8. use 1-2µl from supernatant for PCR (in 20ul reaction)

Alternative methods