Difference between revisions of "Tamoxifen"

Revision as of 00:08, 11 November 2014

This method describes how to induce expression of cre-recombinase in tamoxifen-inducible transgenic Cre-mouse lines.

Tamoxifen from Sigma, catalogue number: T5648.

use either sesame or peanut oil

Weigh in 100mg of tamoxifen
Resuspend the tamoxifen in 1ml of ethanol (100%) and mix; (this step will take some time; if necessary warm to 37ºC for 20-30 minutes)
After tamoxifen is dissolved, add 9ml of sesame or peanut oil
Mix on shaker
Aliquot and store until use at -20ºC

Inject intra-peritoneal (IP) using either of the following dosages:
1. 20mg/kg/day over 6 days,
2. 30mg/kg/day over 5 days, or
3. 80mg/kg/day over 2 (to 3) days.

tamoxifen injection and cre expression may be linked to a transient hypertrophy reaction in mice.
References:

Cre-loxP DNA recombination is possible with only minimal unspecific transcriptional changes and without cardiomyopathy in Tg(alphaMHC-MerCreMer) mice.
Dilated cardiomyopathy resulting from high-level myocardial expression of Cre recombinase.
Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models.

@@ Line 39: / Line 39: @@
 # Cre-loxP DNA recombination is possible with only minimal unspecific transcriptional changes and without cardiomyopathy in [http://www.ncbi.nlm.nih.gov/pubmed/20802136 Tg(alphaMHC-MerCreMer)] mice.
 # Dilated cardiomyopathy resulting from high-level myocardial expression of [http://www.ncbi.nlm.nih.gov/pubmed/16762803?access_num=16762803&link_type=MED&dopt=Abstract Cre recombinase].
+# Avoidance of transient cardiomyopathy in cardiomyocyte-targeted [http://www.ncbi.nlm.nih.gov/pubmed/19520971 tamoxifen-induced MerCreMer gene deletion models.]