Difference between revisions of "Tamoxifen"

Revision as of 00:03, 11 November 2014

This method describes how to induce expression of cre-recombinase in tamoxifen-inducible transgenic Cre-mouse lines.

Tamoxifen from Sigma, catalogue number: T5648.

use either sesame or peanut oil

Weigh in 100mg of tamoxifen
Resuspend the tamoxifen in 1ml of ethanol (100%) and mix; (this step will take some time; if necessary warm to 37ºC for 20-30 minutes)
After tamoxifen is dissolved, add 9ml of sesame or peanut oil
Mix on shaker
Aliquot and store until use at -20ºC

Inject intra-peritoneal (IP) using either of the following dosages:
1. 20mg/kg/day over 6 days,
2. 30mg/kg/day over 5 days, or
3. 80mg/kg/day over 2 (to 3) days.

tamoxifen injection and cre expression may be linked to a transient hypertrophy reaction in mice.
References:

Cre-loxP DNA recombination is possible with only minimal unspecific transcriptional changes and without cardiomyopathy in Tg(alphaMHC-MerCreMer) mice.
Dilated cardiomyopathy resulting from high-level myocardial expression of Cre recombinase.

@@ Line 31: / Line 31: @@
 *# 30mg/kg/day '''over 5 days''', or
 *# 80mg/kg/day '''over 2 (to 3) days'''.
+<br />
+----
-''CAUTION'': tamoxifen injection and cre expression may be linked to a transient hypertrophy reaction in mice.<br />
+=== CAUTION ===
+tamoxifen injection and cre expression may be linked to a transient hypertrophy reaction in mice.<br />
 References:
 # Cre-loxP DNA recombination is possible with only minimal unspecific transcriptional changes and without cardiomyopathy in [http://www.ncbi.nlm.nih.gov/pubmed/20802136 Tg(alphaMHC-MerCreMer)] mice.
 # Dilated cardiomyopathy resulting from high-level myocardial expression of [http://www.ncbi.nlm.nih.gov/pubmed/16762803?access_num=16762803&link_type=MED&dopt=Abstract Cre recombinase].