Difference between revisions of "Tail DNA extraction"

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(Created page with "== Extraction of DNA from mouse tails == === Method Summary === This method describes how to extract DNA from mouse tails. Please see also #Alternative methods|Alternative...")
 
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=== Alternative methods ===
 
=== Alternative methods ===
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<pdf>File:Tail_DNA.pdf</pdf>

Latest revision as of 02:20, 20 November 2014

Extraction of DNA from mouse tails

Method Summary

This method describes how to extract DNA from mouse tails. Please see also Alternative methods.


Materials

Lysis buffer

50mM NaOH

Neutralization buffer

1M Tris pH8



Method

  1. have each tail in a separate eppendorf tube
  2. add 400μl of Lysis buffer to each tail and incubate for 20-30 mins. at approx. 95°C
  3. (vortex 1-2 times in between to help dissolve tissue)
  4. add 50µl of Neutralization buffer
  5. vortex briefly
  6. spin 1 minute max speed in tabletop centrifuge to collect bones and
  7. remaining tissue (hairs) at bottom of the tube
  8. use 1-2µl from supernatant for PCR (in 20ul reaction)


Alternative methods

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