Difference between revisions of "Tail DNA extraction"
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Revision as of 02:03, 18 November 2014
Contents
Extraction of DNA from mouse tails
Method Summary
This method describes how to extract DNA from mouse tails. Please see also Alternative methods.
Materials
Lysis buffer
50mM NaOH
Neutralization buffer
1M Tris pH8
Method
- have each tail in a separate eppendorf tube
- add 400μl of Lysis buffer to each tail and incubate for 20-30 mins. at approx. 95°C
- (vortex 1-2 times in between to help dissolve tissue)
- add 50µl of Neutralization buffer
- vortex briefly
- spin 1 minute max speed in tabletop centrifuge to collect bones and
- remaining tissue (hairs) at bottom of the tube
- use 1-2µl from supernatant for PCR (in 20ul reaction)
Alternative methods
