View source for Passaging of C2C12 cells

==Making a New Passage C2C12 cells==
===Summary===
We passage cells to keep them alive and growing in the incubator in the cell culture room. Generally cells should be passaged every two days

===Materials===
* Cell plates 
*Proliferation media
*PBS 
*Trypsin

===Methods===
# Warm all solutions needed (PBS, prolif media) trypsin is not warmed
# Label new dish (cell type/ passage number/ date/ initials/ prolif) 
# Add 10 ml of prolif media to new plate
# Take up old cell media with vacuum and pipette tips
# Wash old plate with 10 ml of PBS and remove
# Add 1 ml of trypsin and place in incubator for two minutes (or until cells are detached) 
# Once cells are detached flush old plate a few times and take up 100 ul of cells (fridays- 70 ul) and drop five drops onto the dish
# Flush new dish with cells a few times
# Place in incubator, gently shake plate in incubator to spread cells and media