View source for Measuring RNA Concentration

===Summary===
In order to quantify the concentration of RNA we have in our sample we use a Nanodrop 
* Nanodrop is located in the hallway 
* Need kimwipes and milli Q water (MQ water is by the machine) 
* The nanodrop program is called “nanodrop 2000” and its located on the desktop
* Use only 1 ul of solution on metal so use a small volume pipette
===Using a nanodrop===
# Clean the nanodrop with MQ water, make sure to thoroughly dry 
# Click “nucleic acid”
# Click “no”
# Click “okay for verification” 
# Load blank solution (MQ water)
# Set pipette to 1 ul and take up water. Place on sensor as a small drop in the middle of the sensor 
# Hit the “blank” button (only have to do blank once in the beginning
# Clean with MQ water and dry
# Measure the concentration of MQ water again to test (should give a value of zero) 
# Hit “measure”
# “Save data in folder”
# Repeat steps with each tube and save data. Change tips between each sample. Make sure program says “RNA” 
# Record concentrations in your lab book and on the tube
# On the tube write “Ɣ (gamma) = ng/ul”
# Also record the 260/280 and 260/230 counts