View source for Making 2% Agarose Gel for PCR Analysis

==Making 2% Agarose Gel for PCR Analysis==
===Materials===
* Agarose powder (kept in cabinet) 
* 1x TAE liquid (by sink)

===Methods===
# Start by taping the gel mold (found behind sink) , use three layers of tape each end 
# Place in the gel mold combs, make sure they are all facing the same direction (knobs towards you) 
# Create the gel solution in the agarose gel glass beaker
* 250 ml TAE
* 5g of agarose powder
# Microwave for two minutes, swirl carefully (make sure you have the rubber holder) if not dissolved microwave for another minute. Then let it cool
# Once dissolved and cooled add 25 ul of 10,000x DNA stain solution (kept in fridge door_brown tube) and swirl until orange color disappears 
# Pour solution into agarose mold (in the hood) slowly from one side, and set to solidify (for a quick solid place in cold room)
# Once solid cut gels and store in black fridge in the gel container