View source for Creating Cell Culture Media

==How to Make Cell Culture Media for C2C12 and Satellite Cells==
===Differentiation Media for C2C12 cells===
* 200 ml DMEM
* 4 ml horse serum (2%)
* 3 ml penicillin/ streptomycin
# Use a sterile vacuum filter (found on bench) 
# In the hood, place all ingredients for the media in the top half of the filter 
# Attach the vacuum filter hose to increase filtration 
# Detach hose, label new bottle (name/date/ recipe)  
# Store in fridge 

===Proliferation Media for C2C12 and Satellite cells===
* 250 ml DMEM
* 50 ml FBS (fetal bovine serum- includes growth factors) 
* 3 ml penicillin/streptomycin
# Use a sterile vacuum filter (found on bench)
# In the hood, place all ingredients for the media in the top half of the filter
# Attach the vacuum filter hose to increase filtration
# Detach hose, label new bottle (name/date/ recipe) 
# Store in fridge 

===Proliferation Media for Satellite cells===
* 250 ml Harms 
* 50 ml FBS (fetal bovine serum- includes growth factors) 
* 3 ml penicillin/ streptomycin
# Use a sterile vacuum filter (found on bench)
# In the hood, place all ingredients for the media in the top half of the filter
# Attach the vacuum filter hose to increase filtration
# Detach hose, label new bottle (name/date/ recipe) 
# Store in fridge